|Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH+
|SeqFISH+ can image mRNAs for 10,000 genes in single cells-with high accuracy and sub-diffraction-limit resolution-in the cortex, subventricular zone and olfactory bulb of mouse brain, using a standard confocal microscope.
|Wild-type mice C57BL/6J of post-natal day (P)23 (male) and P40 (male) were used for the cortex and olfactory bulb seqFISH+.
|913 cortex cells, 2050 olfactory bulb cells
|RNA-seq data were obtained from GEO accession number GSE98674. RNA SPOTs data were obtained from a previous study. Source data are available at https://github.com/CaiGroup/seqFISH-PLUS.